5-Aminolaevulinate hydro-lyase from yeast isolation and purification
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Purification and properties of D-4-deoxy-5-oxoglucarate hydro-lyase (decarboxylating).
1. An enzyme extracted from Pseudomonas acidovorans was purified and shown to catalyse the simultaneous dehydration and decarboxylation of d-4-deoxy-5-oxoglucarate. It is proposed to name the enzyme d-4-deoxy-5-oxoglucarate hydro-lyase (decarboxylating), trivial name ;deoxyoxoglucarate dehydratase'. 2. No added cofactors were required, and the enzyme was inactivated when incubated with its subs...
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Yeast phenylalanine ammonia-lyase (EC 4.3.1.5) catalyzes the deamination of L-phenylalanine to form trans-cinnamic acid and tyrosine to trans-coumaric acid. Maximal enzyme activity in Rhodotorula glutinis (2 units/g, wet weight, of yeast) was induced in late-log phase (12 to 14 hours) of growth in a culture medium containing 1.0% malt extract, 0.1% yeast extract, and 0.1% L-phenylalanine. A hig...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Enzymology
سال: 1967
ISSN: 0005-2744
DOI: 10.1016/0005-2744(67)90051-4